Objective To develoP an atomic fluorescence spectrometric method for detecting the concentration of antimony in urine.
Methods Urine samples were digested using mixed acids and treated with thiourea and ascorbic acid to reduce pentavalent antimony to trivalent antimony.Then potassium borohydride was added to generate stibine which was afterwards decomposed to atomic antimony in quartz furnace atomizer using argon gas.Fluorescence was excited by an incident light from a hollow cathode lamp,and fluorescence intensity was compared with relevant standards to quantify antimony.
Results A good linear relationship for antimony was found in the range of 0.0-10.0 μg/L,and the correlation coefficients were 0.999 4-0.999 9.The lowest limit of detection was 0.06 μg/L;the relative standard deviations were 1.9%-2.4%;the average recovery was 99.7%.
Conclusion The wet digestion hydride generation atomic fluorescence spectrometric method for determination of urine antimony is practical,fast,accurate,highly sensitive,less interfered and easy to promote.Therefore,it is recommended for mass detection of urine antimony in occupational health surveillancegroup
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