Objective To assess the damage to alveolar macrophages (AM) treated by nanometer silica and blocked tumor necrosis factor receptor-associated factor 2(TNFR2),and to explore the role of TNFR2 in inositol requiring enzyme 1(IRE1)-c-Jun N-terminal kinase (JNK) signaling pathway induced AM apoptosis.
Methods AM were divided into blank control group,silica group (50 mg/mL),and anti-TNFR2 group (10 μg/mL anti-TNFR2 antibody was added to the silica group).After cultured for 24 h,AM apoptosis rate and the contents of TNFR2,apoptosis signal-regulating kinase 1 (ASK1),JNK,transcription factor activator protein 1(AP1),Caspase12 were detected.
Results Compared with the blank control group,the silica group showed higher levels of AM apoptosis rate,TNFR2,ASK1,JNK,AP1,and Caspase12.The levels of above indicators were lowered after anti-TNFR2 treatment compared with those of the silica group,but still higher than those of the blank control group.
Conclusion TNFR2 may play apotential important role in JNK-IRE1 signaling pathway induced AM apoptosisgroup
DownLoad: