Objective To examine the oxidative DNA damage induced by titanium dioxide nanoparticles in mice.
Methods Twenty female mice were randomly divided into four groups and administered with titanium dioxide nanoparticles (0,100,200,400 mg/kg body weight) by intravenous injection.All the mice were sacrificed at the 24 h and samples of liver,lung,kidney,bone marrow,and brain were harvested for 8-hydroxyguanosine (8-OHdG) detection.
Results The counts of 8-OHdGper 106 dG in liver DNA of three titanium dioxide nanoparticle-treated groups (1.07& #177;0.11,1.49& #177;0.13,and 1.39& #177;0.18) wereobviously higher as compared with the counts of the control group (0.82& #177;0.06)(P < 0.01).No change of 8-OHdG level was observedin the other tested tissues (P > 0.05).
Conclusion Titanium dioxide nanoparticles could elevate the oxidative DNA damage inmice liver but not in lung,kidney,bone marrow,and braingroup
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