Objective To establish a simultaneous multi-agent measurement method by high performance lipid chromatography to determine benzoic acid, sorbic acid, saccharin sodium, and acesulfame in wine.
Methods Samples were heated by water bath to remove ethanol, adjusted pH to 7.0, and filtered to perform liquid chromatography, using retention time for qualification and peak area for quantification.
Results Effective simultaneous separation was achieved of benzoic acid, sorbic acid, saccharin sodium, and acesulfame. The method's detection limits were 1.6 mg/kg for sorbic acid, 2.0 mg/kg for benzoic acid, 2.0 mg/kg for saccharin sodium, and 2.5 mg/kg for acesulfame. A good linearity was found within 1.0-50.0 μg/mL, with correlation coefficient >0.999, relative standard deviation of 1.6%-3.4% (n=6), and recovery rate of 92.0%-108.6%.
Conclusion The sensitive, simple, rapid, accurate, and reproducible method is suitable for the determination of sorbic acid, benzoic acid, saccharin sodium, and acesulfame in wine simultaneously
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