Objective To evaluate the genotoxicity of sub-chronic exposure to lanthanum chloride on mice using micronucleus test and comet assay.
Methods SPF grade ICR mice (n=100) were randomly divided into five groups,includingfour lanthanum chloride exposure groups and a control group,20 mice in each group,male and female half and half.The five dose groups were fed by gavage respectively with different concentrations of lanthanum chloride solution (0,10,20,50,and 100 mg/kg),6 times a week,for 13 consecutive weeks.Peripheral blood samples of mice were collected for comet assay to determine lanthanum content,and bone marrow samples were taken to observe bone marrow micronucleus rate.
Results The comet tail length of the cells and the tail DNA content in the groups exposed to lanthanum 50 mg/kg and up were significantly different compared with the control group (P < 0.05),so were mouse bone marrow micronucleus rates (P < 0.05).The four lanthanum chloride treated groups showed varied degrees of lanthanum accumulation in blood,and showed significant differences compared with the control group (P < 0.05).
Conclusion Lanthanum could accumulate in blood and lead to genetic toxicitygroup
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