Objective To observe the antagonistic effect of grape seed proanthocyanidin extract (GSPE) on oxidative damage of male mice which were treated with excessive sodium fluoride (NaF).
Methods A total of 40 healthy and sexually mature male mice were randomly divided into four groups:control,NaF (20 mg/kg),GSPE (200 mg/kg),and NaF (20 mg/kg)+GSPE (200 mg/kg) groups.The animals were fed with deionized water supplemented with non-NaF,NaF,GSPE,or NaF+GSPE for consecutive five weeks,and sacrificed by cervical dislocation to collect liver tissue samples.We calculated the liver/body weight index and measured the activities of alanine aminotransferase (ALT),aspartate aminotransferase (AST),and superoxide dismutase (SOD) and the levels of glutathione (GSH),malondialdehyde (MDA),and total anti-oxidant capacity (T-AOC).
Results The differences in liver/body weight index were statistically significant among the experiment groups (P < 0.05).Compared with the control group,the liver/body weight index of the NaF group was increased;compared with the NaF group,that of the NaF+GSPE group was decreased.The differences in AST and ALT activities among the experiment groups were statistically significant (all Ps < 0.05).Compared to the control group,the activities of AST and ALT of the NaF group and the NaF+GSPE group were increased (P < 0.05);compared to the NaF group,the GSPE group and the NaF+GSPE group were decreased (P < 0.05).The differences in GSH,SOD,MDA,and T-AOC contents among the experiment groups were also statistically significant (all Ps < 0.05).Compared with the control group,the contents of GSH,SOD,and T-AOC of the NaF group and the NaF+GSPE group were decreased (P < 0.05),but the MDA content was increased (P < 0.05);compared with the NaF group,the contents of GSH,SOD,and T-AOC of the GSPE group and theNaF+GSPE group were increased (P < 0.05),but the MDA content was decreased (P < 0.05).The results of factorial analysis showed an antagonistic interaction between NaF and GSPE on AST,ALT,SOD activities,MDA content,and T-AOC level.
Conclusion GSPE could antagonize the oxidative damage induced by excessive NaF in male micegroup
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