Urinary 8-OHdG Levels and Related Impact Factors in General Population

Objective To measure urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in general population and assess the roles of potential impact factors.

Methods The urinary levels of 8-OHdG of 257 urban residents in a city of east China were measured with ultra-high performance liquid chromatography-mass spectrometer, and creatinine by high performance liquid chromatography to adjust the urinary 8-OHdG levels. The impact factors were analyzed by multivariate linear regression models, and interaction effects by multivariate analysis of variance.

Results The median (P₂₅~P₇₅) level of 8-OHdG in local general population was 1.50 (0.39-2.24) μg/L, and the level adjusted by creatinine in urine was 129.82 (50.79-235.58) μg/mol. We did not find changes of urinary 8-OHdG by smoking, drinking, and body mass index (BMI) alone after stratification analyses. By multivariate linear regression analysis, we found that drinking had a significant effect on the urinary levels of 8-OHdG (P=0.040) after controlling other selected confounding factors. And we found an increasing trend of urinary levels of 8-OHdG with older age in the overweight group (P_trend < 0.05).

Conclusion The interaction between age and BMI might be an important impact factor for urinary levels of 8-OHdG. Overweight individuals in older age group tend to be at a higher risk of DNA oxidative damage.