Objective To establish determination methodology of pentachlorophenol in fish gall bladder by automatic solid-phase microextraction(SPME)with ancillary extraction of supersonic wave and gas chromatography.
Methods Pentachlorophenol in fish gall bladder was extracted using optimized SPME technology, separated by HP-5 and DB-17 chromatographic columns and the content of pentachlorophenol in fish gall bladder was determined by electron capture detector(ECD)under optimized conditions.
Results The linear range of the calibration curve was 0.08-100.00 μg/L with a correlation coefficient of 0.9997 and the detection limit for pentachlorophenol was 0.053 μg/L. The recovery rates were between 81.5%-92.6% and the relative standard deviations were between 3.6%-6.3%.
Conclusion The method is simple, rapid and sensitive without solvent pollution, which is a ideal method for determination of pentachlorophenol in fish gall bladder.
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