Objective To investigate the prevalence and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) in hospital, and to provide scientific basis for the prevention and control of MRSA nosocomial in fection.
Methods Totally 78 strains of Staphylococcus aureus (SA) were isolated from various clinical specimens and 53 strains of SA were isolated from hospital environment in the same time period in a secondary general hospital in Shanghai. MRSA was detected by cefoxitin disk diffusion test and polymerase chain reaction. The antimicrobial susceptibility of SA was tested by K-B disk diffusion test against 15 antibiotics agents.
Results Among the 131 strains, the proportion of MRSA was 55.73% (73/131); the proportion of MRSA in the clinical strains and the environmental strains were 52.56% and 60.38% respectively (P>0.05). The clinical MRSA strains were mainly distributed in sputum and wound secretion, and the environmental MRSA strains were mainly distributed in bedside tables, patient monitors, and the hands of medical staff. The MRSA-to-& #223;-lactam antibiotic resistance rate was 100%; all of the MRSA samples were highly sensitive to glycopeptide drugs, linezolid, rifampin, and showed multidrug resistance to other antibiotics.
Conclusion MRSA is highly prevalent in hospital, and most MRSA strains are multi-drug resistant. Rational use of antibiotics and surveillance of MRSA nosocomial infection should be strengthened. Disinfection and quarantine for patients in fected by MRSA should be performed in time.
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