Objective To investigate the toxic effects of aluminum on primary cultured neurocytes in mice and the metabotropic glutamate receptor 3 (mGluR3) expression.
Methods The neurocytes were treated with aluminum chloride (AlCl3& #183;6H2O)at final concentrations of 0 mmol/L(control), 0.5 mmol/L(low-dose), 1.0 mmol/L(medium-dose), and 2.0 mmol/L(high-dose) separately; then incubated for 48 hour. The CCK-8 enzyme-linked immunosorbent assay kit was used to detect celt viability, and the expression ofmGluR3 were detected by RT-PCR.
Results Compared with control group, AlCl3 decreased the viability of cultured neurocytes in high dose group significantly(P<0.05). The RT-PCR demonstrated that mGluR3 gene expression in high dose group decreased significantly compared with control group after aluminum exposure(P<0.05).
Conclusion The cytotoxic effect of aluminum on primary cultured neurocytes in mice may be associated with the decrease of mGluR3 gene expression.
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