氢醌对TK6细胞周期的影响及其相关的调控机制探讨

刘林华; 陈玉婷; 唐焕文; 张贺; 程小广; 杨慧; 凌晓璇

doi:10.13213/j.cnki.jeom.2014.0035

氢醌对TK6细胞周期的影响及其相关的调控机制探讨

Effects of Hydroquinone on TK6 Cell Cycle and Related Regulatory Mechanism

摘要

摘要:
目的探讨氢醌(hydroquinone,HQ)对细胞周期的影响及其相关的调控机制。

方法磷酸盐缓冲液(PBS)处理人类淋巴母细胞(TK6细胞)为对照组,20.0 μmol/L HQ为处理组,48 h后通过碘化丙啶(PI)标记的流式细胞术检测细胞周期分布情况,用实时荧光定量聚合酶链反应(qRT-PCR)检测PTEN (抑癌基因)、p53基因mRNA表达的改变,流式细胞荧光标记法检测Rb蛋白的表达。

结果与对照组比较,处理组G₁期细胞比例明显增加,S期细胞比例明显减少,差异均有统计学意义(P<0.05);HQ作用于TK6细胞后上调了Rb的表达,下调了p53的表达,其差异均有统计学意义(P<0.05),而对PTEN的表达没有明显影响。

结论 HQ可能通过上调Rb的表达使细胞阻滞于G₁期。

Abstract:
Objective To investigate the effects of hydroquinone (HQ) on cell cycle and related regulatory mechanismin TK6 human lymphoblasts cells.

Methods Phosphate buffered solution (PBS)-treated TK6 cells were selected as the controlgroup,and 20 μmol/L HQ-treated same line cells as the HQ-treated group.The distribution of cell cycle was determined with a flowcytometry kit using propidium iodide (PI) staining,the mRNA expressions of PTEN and p53 were quantified by reverse transcriptionreal-time polymerase chain reaction assay,and Rb protein expression was measured by flow cytometry.

Results Compared with thecontrol group,the proportion of G₁-phase cells in the HQ-treated group was increased,while the proportion of S-phase cells was decreased,and significant differences in the above indicators were observed between the two groups (P<0.05).Up-regulated expression of Rb proteinand down-regulated expression of p53 mRNA were observed in the HQ-treated group (P<0.05),but no significant effect on the expressionof PTEN mRNA was observed.

Conclusion HQ may arrest TK6 cells at G₁ phase by raising the expression of Rb protein.

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