氯化汞对睾丸间质细胞类固醇激素合成影响的体外研究

高慧艳; 李江敏; 王燕; 陈小玉

doi:10.13213/j.cnki.jeom.2015.15206

氯化汞对睾丸间质细胞类固醇激素合成影响的体外研究

In Vitro Effects of Mercury on Steroid Hormone Biosynthesis of Leydig Cells

摘要

摘要:
目的探讨氯化汞(HgCl₂)对SD大鼠体外培养睾丸间质细胞(leydig cell)类固醇激素合成的影响。

方法不同浓度HgCl₂ (1& #215;10^-8、1& #215;10^-7、1& #215;10^-6、1& #215;10^-5 mol/L)作用于leydig 细胞后, 测定孕酮含量, 运用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测HgCl₂ 对leydig 细胞中StAR、P450scc 和3β-HSD mRNA表达的影响。

结果经不同浓度HgCl₂ 染毒后, leydig 细胞分泌孕酮的量均有所降低, 其中1& #215;10^-7、1& #215;10^-6、1& #215;10^-5 mol/L HgCl₂ 染毒组与对照组相比, 差异均有统计学意义(P < 0.05)。1& #215;10^-7、1& #215;10^-6 和1& #215;10^-5 mol/L HgCl₂ 剂量组的StAR、P450scc 和3β-HSD mRNA相对表达量均低于对照组(P < 0.05)。

结论汞可以影响睾丸间质细胞类固醇激素的合成。

Abstract:
Objective To assess the effects of mercury chloride (HgCl₂) on steroid hormone biosynthesis in leydig cells isolated from SD rat testis.

Methods Leydig cells were exposed to various concentrations of HgCl₂ (1& #215;10^-8, 1& #215;10^-7, 1& #215;10^-6, and 1& #215;10^-5 mol/L). Progesterone in the supernatant was measured. The levels of mRNA expression of StAR, P450scc, and 3β-HSD in leydig cells were determined by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR).

Results The progesterone content secreted by Leydis cells was reduced after treated by various concentrations of HgCl₂, and the progesterone contents in the 1& #215;10^-7, 1& #215;10^-6, and 1& #215;10^-5 mol/L HgCl₂ groups were significantly lower than that of the control group (P < 0.05). The mRNA relative expression levels of StAR, P450scc, and 3β-HSD in the leydig cells exposed to HgCl₂ at the concentration of 1& #215;10^-7, 1& #215;10^-6, and 1& #215;10^-5 mol/L were also statistically lower than that of the control group (P < 0.05).

Conclusion Mercury could affect steroid hormone biosynthesis of leydig cells.

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