氡暴露致小鼠肺组织线粒体转录因子A及核因子-κB表达的改变

裴炜炜; 陶立静; 聂继华; 童建

doi:10.13213/j.cnki.jeom.2016.15434

氡暴露致小鼠肺组织线粒体转录因子A及核因子-κB表达的改变

Alteration in Expression of Mitochondrial Transcription Factor A and Nuclear Factor-κB in Mouse Lung Tissue Exposed to Radon

摘要

摘要:
目的观察氡对小鼠肺组织线粒体转录因子A（TFAM）及核因子（NF）-κB表达的影响，为探讨氡致肺损伤的机制提供实验依据。
方法 20只健康雌性BALB/c小鼠随机分为对照组及3个染氡组，每组5只，染氡组氡暴露剂量分别为30、60、120工作水平月。末次染毒后1 h内处死小鼠，取肺组织进行HE染色以及TFAM和NF-κB p65免疫组化染色，Western blot检测肺组织中TFAM和NF-κB p65的蛋白表达。
结果免疫组化结果显示，染氡后TFAM、NF-κB p65阳性细胞数显著增加，其中120工作水平月组的TFAM和NF-κB p65阳性细胞率（0.521±0.081）%、（0.260±0.068）%高于对照组（0.093±0.011）%、（0.069±0.016）%，P < 0.05，Western blot结果显示其蛋白表达也显著上升。
结论氡暴露小鼠肺组织中TFAM和NF-κB的表达上调，提示NF-κB的信号通路被激活。

Abstract:
Objective To observe changes in expression of mitochondrial transcription factor A (TFAM) and nuclear factor kappa B (NF-κB) in mouse lung tissue induced by radon, so as to provide experimental evidence for understanding related mechanism.
Methods Twenty healthy female BALB/c mice were randomly divided into one control group and three experimental groups with cumulative doses of radon at 30, 60, and 120 working level months (WLM). All mice were sacrificed 1 h after last treatment. The lung tissues were stained with HE, and the expressions of TFAM and NF-κB p65 were detected by im munohistochemistry and Western blot.
Results The immunohistochemistry results showed that the positive cell rates of TFAM and NF-κB p65 significantly increased after radon exposure, and the 120 WLM group presented higher positive cell rates of TFAM and NF-κB p65 (0.521±0.081)%, (0.260±0.068)% than the control group (0.093±0.011)%, (0.069±0.016)%, P < 0.05. So did the expression of protein according to Western blot.
Conclusion The elevated expressions of TFAM and NF-κB induced by radon indicate an activation of NF-κB signaling pathway.

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