Comparative Study on Curative Effects of Pulmonary Perfusion with Ulinastatin and Ambroxol on Lung Injury Induced by Paraquat in Rabbits
30只家兔随机分为5组：百草枯染毒组、生理盐水灌洗组、氨溴索灌洗组、乌司他丁灌洗组及对照组，每组6只动物。前4组一次性灌胃50 mg/kg体重的百草枯，3个灌洗组在染毒24 h后，分别给予生理盐水、氨溴索、乌司他丁进行肺灌洗；对照组一次性灌胃等体积的生理盐水，24 h后再行生理盐水肺灌洗。采集各组动物染毒前、染毒后第1、3、7天血液进行血清学检测；染毒后第7天处死动物，取其右肺组织进行病理学观察。
P<0.05）。染毒后第7天，氨溴索灌洗组血清MPO及IL-1含量低于生理盐水灌洗组（均 P<0.05）；氨溴索灌洗组HYP水平高于乌司他丁灌洗组（ P<0.05），但低于生理盐水灌洗组（ P<0.05）；乌司他丁灌洗组TNF-α水平低于氨溴索灌洗组（ P<0.05）。3个灌洗组肺组织病理改变较染毒组轻。 结论
To compare the curative effects of selected lung lavage fluids on rabbits'lung injury induced by paraquat (PQ).
Thirty rabbits were randomly assigned to PQ group,normal saline group,ambroxol group,ulinastatin group,and control group,with six rabbits per group.Rabbits of the exposure groups were intragastrically administered once with PQ at 50 mg/kg;the normal saline,ambroxol,and ulinastatin groups were treated by lung lavage with respective agents after 24 h exposure of PQ;animals in the control group were administered with 50 mg/kg physiological saline and treated by lung lavage with saline after 24 h.Before exposure and at 1,3&7 d after exposure,rabbit auricular vein blood was sampled for serum examination.The animals were executed on the 7th day after exposure and right lung tissues were sampled for pathological observation.
On the 3rd and 7th day after exposure,the levels of serum myeloperoxidase (MPO),hydroxyproline (HYP),interleukin 1(IL-1),and tumor necrosis factor α(TNF-α) of the normal saline,ambroxol,and ulinastatin groups were significantly lower than those of the PQ group (all
Ps<0.05).On the 7th day after exposure,the MPO level and IL-1 level were lower in the ambroxol group than those in the normal saline group (both Ps<0.05);the HYP level in the ambroxol group was higher than that in the ulinastatin group ( P<0.05),but lower than that in the normal saline group ( P<0.05);the TNF-α level in the ulinastatin group was lower than that in the ambroxol group ( P<0.05).Milder pathological changes were found in the three lung lavage groups than those in the PQ group. Conclusion
Lung lavage might have protective effects on the inflammatory response of acute lung injury caused by PQ in rabbits.Lung lavage with ambroxol or ulinastatin is better than using physiological saline alone.