铝对apoE基因敲除小鼠认知能力及相关蛋白的影响

The Effect of Aluminum Chloride on Cognitive Ability and Related Protein in apoE Knock Out Mice

  • 摘要:
    目的 研究三氯化铝侧脑室注射对 apoE基因敲除小鼠空间学习和记忆能力的影响及相关蛋白代谢型谷氨酸受体 -1(mGluR1)的表达。

    方法 取经跳台试验、避暗试验和 Morris水迷宫试验筛选的正常 apoE基因敲除小鼠,用随机数字表法按体重随机分为 2组:对照组和 0.5%三氯化铝染铝组, 每组 8只。染铝组经侧脑室注射染毒, 3 μL/d, 连续 5 d; 对照组以相同容量生理盐水注射, 染毒期结束后从第 15天开始用跳台试验、避暗试验和 Morris水迷宫试验判断 2组的认知能力; 染毒期结束 20 d后断头取脑组织, 用 Western-blot方法对 mGluR1蛋白进行定量检测。

    结果 ①染铝组跳台和避暗潜伏期较对照组明显缩短(P < 0.05); 第 2天错误次数明显增多(P < 0.05); Morris水迷宫试验结果显示,染铝组找到平台的潜伏期较对照组明显增加(P < 0.05), 而空间探索试验中在目标象限停滞时间明显减少(P < 0.05)。②染铝组 mGluR1表达量较对照组降低, 且差别有统计学意义(P < 0.01)。

    结论 铝可致 apoE基因敲除小鼠学习和记忆能力障碍, 且可使 mGluR1蛋白的表达降低。

     

    Abstract:
    Objective To investigate the effects of aluminum chloride(AlCl3), which was administrated by lateral cerebral ventricle injection, on spacial learning and memory in apoE knock out mice, as well as their expression of metabotropic glutamate receptor-subtype 1(mGluR1).

    Methods After being successfully trained by step through test, step down test and morris water maze(MWM), 16 mice were evenly and randomly divided into 2 groups:control group and exposed group. Mice in the latter group were treated with 0.5% AlCl3 by lateral ventricle injection for 5 d, 3μL/d, while the controls were treated with normal saline in the same volume as the exposed group. Cognitive ability was evaluated by step through test, step down test and MWM on the 15th day after Al treatment for 5 d. Cerebral cortex was taken from the mice on 20th day after 5 d Al treatment. mGluR1 in cytoplasm was detected by western-blot, images were collected respectively by gelatum analyzing system and scanner. Data were analyzed by the gelatum analyzing system.

    Results ① Latent period of step down test and step through test in the exposed group were significantly decreased(P < 0.05), the error count was significantly increased in the second day(P < 0.05)compared with the control group. In MWM the time of place navigation was significantly increaseed(P < 0.05), and the time of spatial probe was significantly shortened(P < 0.05)compared with the control group. ② The expression of mGluR1 was significantly decreased in the 0.5% AlCl3 exposed group compared with the control group(P < 0.05).

    Conclusion The learning and memory ability and the expression of mGluR1 in apoE knock out mice are decreased obviously by AlCl3 lateral ventricle injection.

     

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