Objective To investigate the effects of hydroquinone (HQ) on cell cycle and related regulatory mechanismin TK6 human lymphoblasts cells.
Methods Phosphate buffered solution (PBS)-treated TK6 cells were selected as the controlgroup,and 20 μmol/L HQ-treated same line cells as the HQ-treated group.The distribution of cell cycle was determined with a flowcytometry kit using propidium iodide (PI) staining,the mRNA expressions of PTEN and p53 were quantified by reverse transcriptionreal-time polymerase chain reaction assay,and Rb protein expression was measured by flow cytometry.
Results Compared with thecontrol group,the proportion of G1-phase cells in the HQ-treated group was increased,while the proportion of S-phase cells was decreased,and significant differences in the above indicators were observed between the two groups (P<0.05).Up-regulated expression of Rb proteinand down-regulated expression of p53 mRNA were observed in the HQ-treated group (P<0.05),but no significant effect on the expressionof PTEN mRNA was observed.
Conclusion HQ may arrest TK6 cells at G1 phase by raising the expression of Rb protein.
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