Objective To establish a method for the determination of chloroprene in blood by solid phase extraction gas chromatography.
Methods Chloroprene in blood samples was concentrated by C18 column and eluted with methanol. The elution was determined by gas chromatography with ECD detector and quantified by external standard method.
Results The linear range of the proposed method was 0.42-20.0 μg/L, with a correlation coefficient (r) of 0.999 8. The detection limit was 0.42 μg/L. The minimum detectable concentration in the blood samples was 0.42 μg/L. The spiked recovery rate was 82.0%-91.3%.
Conclusion The method is of high sensitivity, easy operation, and high specificity. It can be used for clinical diagnosis and treatment of occupational chloroprene poisoning.
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