Objective To examine the injury to rats induced by microwave radiation in hypergravity setting.
Methods Rats were randomly divided into control, microwave, and hypergravity + microwave groups. There were 12 rats in each group. The hypergravity+microwave group was firstly exposed to a 6G setting for 5 min, and then exposed to 200 mW/cm2 microwave under the 6G environment for another 5 min.
Results Serum nitric oxide (NO) in the control and microwave groups were significantly different from that of the hypergravity+microwave group (P<0.05). The levels of superoxide anion and nitric oxide synthase (NOS) in the microwave and hypergravity+microwave groups were significantly different from those of the control group (q1=13.587 6, q2=25.923 5, P<0.05); similar differences were also found between the microwave group and the hypergravity+microwave group (q=11.487 6, P<0.05). The level of catalase (CAT) in the microwave group was different as compared to those of the control and the hypergravity+ microwave groups (q1=7.840 4, q2=5.339 1, P<0.05). The level of glutathione peroxidase (GSH-pox) in the microwave group was obviously different as compared to that of the control group (q=5.900 1, P<0.05). The level of glutathione (GSH) in the hypergravity+ microwave and the microwave groups were significantly different from that of the control group (q1=8.9487, q2=7.114 5; P<0.05). In the liver homogenate, the levels of CAT and GSH-pox in the microwave and the hypergravity+microwave groups were significantly different as compared to those of the control group (q1=3.381 5, q2=2.970 9; P<0.05); the level of NOS in the hypergravity microwave group was obviously different as compared to that of the microwave and the control groups (q1=4.239 3, q2=4.088 3; P<0.05); the level of GSH in the microwave group was obviously different from those of the hypergravity+microwave and control groups (q1=11.663 4, q2=15.983 1, P<0.05). Liver cells were slightly swollen, intercellular space grew bigger, and liver sinusoid became wider under the microscope observation.
Conclusion The mechanism of microwave radiation injury in hypergravity setting is possibly due to increased superoxide anion.
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