Case-Control Study on Relationship Between Polymorphisms of MicroRNA-30c and MicroRNA-196a2 and Cervical Cancer

Objective  To assess the relationship between polymorphisms of MicroRNA(miR)-30c and miR-196a2 and cervical cancer.

Method  A case-control design was carried out in this study, which included 104 patients with cervical cancer and 186 controls from Yancheng City No.1 People's Hospital from September 2012 to September 2014. The gene polymorphisms of miR-30c rs928508 and miR-196a2 rs11614913 in patients and controls were analyzed by multiplex polymerase chain reaction and multiple ligase detection reaction. The association between identified genotypes and cervical cancer was analyzed by chi-square test.

Result  The genotype of miR-30c rs928508 were 16.3% for GG, 51.9% for AG, and 31.8% for AA in the cases and 29.0% for GG, 47.3% for AG, and 23.7% for AA in the controls, and there were statistical differences between the cases and the controls (P<0.05). Compared with GG genotype, AG or AA genotype was significantly associated with an increased risk of cervical cancer (P<0.05), and the OR was 2.38 for individuals with AA genotype versus those with GG (95%CI: 1.17-4.83). No significant association was found between gene polymorphism of miR-196a2 rs11614913 and cervical cancer (P >0.05).

Conclusion  The miR-30c rs928508 polymorphism may be correlated with cervical cancer.