Objective To develop new antidotes of manganese (Mn).
Methods Mice were injected intraperitoneally with MnCl2(100 mg MnCl2/kg)24 h before they were injected intraperitoneally with chelating agents,including L-Histidine (L-His),ethylene diaminetetraacetic acid (EDTA),Diethyldithiocarbamate (DDTC),meso-2,3-dimercapto succinc acid (DMSA) and N-acetylcysteine (NAC).The effects of those chelating agents on lipid peroxidation (LPO) and concentrations of Mn,Ca,Zn in lung were observed.
Results The Mn injection significantly increased the LPO levels (2.42& #177;0.29) and (2.74& #177;0.33)μmol/(g.prot) respectively as well as the concentrations of Mn (2.60& #177;0.33) and (2.41& #177;0.68)μg/g wet tissue respectively in lung and testes of mice (P<0.05) in 2 days.L-His effectively prevented the increases of above indices.
Conclusion L-His is more effective than the other chelating agents in preventing changes caused by acute exposure to Mn.L-His is a promising antidote to Mn.
DownLoad: