Objective To investigate the potential protective mechanism of sodium salicylate(NaSAL)on the pulmonary damage induced by paraquat(PQ)in rats.
Methods We established rat poisoning model by intragastric administration of 80mg/kg PQ. A total of 72 male SD rats were randomly divided into control group, PQ treated group and PQ plus NaSAL intervention group. Control group were orally administered with an injection of 0.9% NaCl at the volume of 0.005 mL/g of body weight; PQ treated group were made 80 mg/kg PQ orally; PQ plus NaSAL group were given an intraperitoneal injection of 120 mg/kg NaSAL after an orally administration of 80 mg/kg PQ. Animals were sacrificed in batches on days 1, 3, 7, 14 after experiment. Then, the level of nuclear factor of kappa B(NF-κB)was assayed by electrophoresis mobility shift assay(EMSA), while the protein levels of tumor necrosis factor-α(TNF-α), transformin growth factor-β1(TGF-β1)were determined by enzyme-linked immunosorbent assay (ELISA).
Results After 80 mg/kg PQ exposure, the levels of TGF-β1, TNF-α protein expression and the rat lung tissue NF- κB activity increased more remarkably than that of the control group(P<0.05). In comparison with PQ treated group, with the treatment of NaSAL(120 mg/kg), the level of TNF-α, TGF-β1 protein expression and the rat lung tissue NF-κB activity decreased significantly(P<0.05)and trended to normal values.
Conclusion Treatment of NaSAL could decrease the level of TNF-α, TGF-β1 protein expression and the rat lung tissue NF-κB activity. It suggested that NaSAL could alleviate paraquat-induced pulmonary damage in rats to some extent.
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