Objective To explore the relations between genetic polymorphism and susceptibility to gastric cancer in Chinese population,and to study the action of genetic factors in development of gastric carcinoma.
Methods A case-control study was performed in 585 controls without tumor matched to 585 original gastric cancer patients in Nanjing city.The genotypes of CYP2E1,GSTM1,GSTT1,NAT2,ALDH2,MTHFR,XRCCl,IL-1β,VDR and TNF were detected by molecular biological techniques (PCR-RFLP and AS-PCR).Sole gene and gene-gene interactions were analyzed by Logistic regression model,and the multi-gene risk of susceptibility genes were analyzed by a multi-gene risk analysis statistic model proposed by Demchuk E.
Results The genetic risk factors associated with primary gastric cancer susceptibility were CYP2E1(c1/c1),NAT2 phenotype (slow acetylator),MTHFRA1298C (A/C),IL-1β(C/T),NAT2M2(A/A),XRCC1194(T/T),NAT2M1(T/T),VDR TaqI (T/T).The multigene risk analysis showed there was close relation between the OR of polygene combination and the genotype frequency.The OR increased along with the susceptibility gene.
Conclusion This new analyze model is helpful to screen and protect the high-risk group of gastric cancer.
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