Objective To investigate the effects of Praseodymium Chloride (PC) on rates of bone marrow micronuclei and nucleus abnormity in mice,as well as possible interventional effect of sodium selenite.
Methods Five to six-week old Kunming mice of were divided into 12 groups:five exposure groups with doses at 7.5,15,30,60,120 mg/(kg& #183;bw) of PC,five treatment groups with the same five PC doses added 0.05 mg/(kg& #183;bw) Selenium respectively,negative (0.85% normal saline) and positive (sodium nitride,20 mg/(kg& #183;bw) groups.All doses were exposed via intraperitoneal injection and standard micronucleus test and nuclear test were followed.Mouse marrow cell frequency micronucleus (FMN) and frequency abnormal nuclei (FAN) were counted and the impacts of FMN and FAN after being added selenite sodium were observed.
Results The 7.5-120 mg/(kg& #183;bw) doses of PC induced a remarkable increase of FMN and FAN and showed a significant correlation between the dosages and their effects (rFMN=0.885,rFAN=0.914).In the pairwise comparison of the treatment groups and the exposure groups,the FMN and FAN decreased after adding sodium selenite (t-test,P<0.05 except in the 7.5 mg/(kg& #183;bw) PC groups).
Conclusion FMN and FAN of mouse marrow cell nucleus increase by exposed to PC,which indicates PC with possible genetic damage effects.Furthermore,sodium selenite could have intervention effects to PC exposure by reducing FMN and FAN.
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