Objective To investigate gene polymorphisms of Interle ukin-12B (IL-12B+1188A/C) and susceptibility of coal worker's pneumoconiosis (CWP) in Han ethnic group workers.
Methods SNP 1188A/C of IL-12B gene was detected by polymerase chain reaction (PCR) based on restriction fragment length polymorphism (RFLP) technique in 120 CWP patients and 120 coal dust exposure workers.
Results The length of exposure to dust,job title,age of smoking frequencies were not significantly different between the cases and the controls (P>0.05).Three genotypes (AA,AC,CC) were detected in IL-12B (1188A/C) and no significant difference was found between the CWP group and the controls (P>0.05) after age standardization.The proportion of C allele showed statistical difference between the cases (45.8%) and the controls (36.7%).Significant difference between the CWP patients and the controls was found in frequencies of C alleles (P<0.05).The distributions in the dust exposure duration,job title and smoking were no significant differences (P>0.05).
Conclusion The study found that IL-12B+1188A/C sites C allele may be one of the susceptible gene in CWP of Han ethnic group in Ningxia area.
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