Objective To guide the design of protection system for biosafety laboratory level-2(BSL-2) with negative air pressure.
Methods The suggestions were proposed through summarized the significant problems in laboratory construction,experiment process,function isolation,floor layout,organization of people and logistics,ventilation,air flowand other environment parameters.
Results Buffer room and dressing room should be included in BSL-2 as well as preparation room.The form of animal room should be regulated and should apply co-current system.The entirely current should flow from pristine area to animal room and air current in animal room should flow form relative cleaning to pollution zone.Baric gradient should not be less than 10Pa.The value of negative pressure should be-20 Pa in core room included combination animal room and-30 Pa in the independence animal room.
Conclusion The design must consistent with biosafety and requirement of experiment process but also should agree with the need of heating ventilation and construction principle.In the paper,the proposed suggestions can guide the construction of laboratory and have been confirmed in the many projects.
DownLoad: